Protein Structural Analysis by Cryogenic Electron Microscopy

Michael Hall; Erin Schexnaydre; Camilla Holmlund; Marta Carroni

doi:10.1007/978-1-0716-3147-8_24

Protein Structural Analysis by Cryogenic Electron Microscopy

Methods Mol Biol. 2023:2652:439-463. doi: 10.1007/978-1-0716-3147-8_24.

Authors

Michael Hall¹, Erin Schexnaydre², Camilla Holmlund², Marta Carroni³

Affiliations

¹ SciLifeLab Cryo-EM Facility, Department of Chemistry, Umeå University, Umeå, Sweden. michael.hall@umu.se.
² SciLifeLab Cryo-EM Facility, Department of Chemistry, Umeå University, Umeå, Sweden.
³ SciLifeLab Cryo-EM Facility, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden. marta.carroni@scilifelab.se.

PMID: 37093490
DOI: 10.1007/978-1-0716-3147-8_24

Abstract

Cryogenic electron microscopy (cryo-EM) is constantly developing and growing as a major technique for structure determination of protein complexes. Here, we detail the first steps of any cryo-EM project: specimen preparation and data collection. Step by step, a list of material needed is provided and the sequence of actions to carry out is given. We hope that these protocols will be useful to all people getting started with cryo-EM.

Keywords: Cryo electron microscopy; Protein structure; Single particle analysis; Vitrification.

MeSH terms

Cryoelectron Microscopy / methods
Humans
Proteins*
Specimen Handling* / methods

Substances

Proteins