The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.
Keywords: Baculovirus-infected insect cells (BIIC); Cell culture; G protein-coupled receptors; Insect cells; Protein Production Sweden (PPS); Protein complexes; Protein kinases; Structural biology.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.