Collagen9a1c localizes to collagen fibers called actinotrichia in zebrafish fins

Hibiki Nakagawa; Toshihiro Aramaki; Shigeru Kondo; Junpei Kuroda

doi:10.17912/micropub.biology.000747

Collagen9a1c localizes to collagen fibers called actinotrichia in zebrafish fins

MicroPubl Biol. 2023 Apr 7:2023:10.17912/micropub.biology.000747. doi: 10.17912/micropub.biology.000747. eCollection 2023.

Authors

Hibiki Nakagawa¹, Toshihiro Aramaki¹, Shigeru Kondo¹, Junpei Kuroda¹

Affiliation

¹ Graduate school of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.

Abstract

Teleost fish fins are supported by spear-shaped collagen crystals called actinotrichia. Actinotrichia are distributed radially at the distal end of the fins and thought to be necessary for proper formation of the fin and fin-bones. We previously reported that collagen9a1c ( col9a1c ) gene product is essential for the regular arrangement of actinotrichia using col9a1c -knockout zebrafish. Here, we examined the localization pattern of the EGFP-tagged Col9a1c protein in the fins to understand its role in the arrangement of actinotrichia. We found that EGFP-Col9a1c specifically localizes to actinotrichia.

Grants and funding

This work was supported by JST CREST, Grant Number 12101628, JSPS KAKENHI, Grant Number 20H05943, JSPS KAKENHI, Grant Number 21H00327, and JST SPRING, Grant Number JPMJSP2138.