Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Amara Dar; Muhammad Nayab Ahmad; Ghufrana Samin; Muhammad Muzammil Jahangir; Rabia Rehman; Jamil Anwar; Zahrah T Al-Thagafi; Zelalem Meraf; Mustafa Musa Jaber

doi:10.1155/2023/9914633

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Int J Anal Chem. 2023 Apr 12:2023:9914633. doi: 10.1155/2023/9914633. eCollection 2023.

Authors

Amara Dar¹, Muhammad Nayab Ahmad¹, Ghufrana Samin², Muhammad Muzammil Jahangir³, Rabia Rehman⁴, Jamil Anwar^{1

5}, Zahrah T Al-Thagafi⁶, Zelalem Meraf⁷, Mustafa Musa Jaber⁸

Affiliations

¹ Centre for Analytical Chemistry, School of Chemistry, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
² Department of Basic Sciences and Humanities, University of Engineering and Technology-Lahore, Faisalabad Campus, Faisalabad, Pakistan.
³ Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan.
⁴ Centre for Inorganic Chemistry, School of Chemistry, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.
⁵ Chemistry Department, University of Management & Technology, Lahore, Punjab, Pakistan.
⁶ Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
⁷ Department of Statistics, Injibara University, Injibara, Ethiopia.
⁸ Department of Medical Instruments Engineering Techniques, Al-Farahidi University, Baghdad 10021, Iraq.

Abstract

A novel pressurized flow system for circular thin-layer chromatography (PC-TLC) has been successfully established and employed for the separation of amino acids, dyes, and pigments for safe medical imaging applications. In this system, the mobile phase is applied to a regular TLC plate through the tube and needle of an intravenous infusion set. The needle was fused in a hole underneath the center of the plate, while the second side end of the tube was connected to a microburette containing the solvent. This new assembly proved itself better in terms of separation time (within 5 minutes) and controlled flow of the solvent and horizontal movement of analyte components over chromatograms with better separation and R _f values (glutamine: 0.26, valine: 0.44, phenylalanine: 0.60, chlorophyll a: 0.52, chlorophyll b: 0.43, xanthophyll: 0.18, carotenoid: 0.97, and pheophytin: 0.60) when a number of samples of amino acids, dyes, and pigments were separated by the developed apparatus and the conventional TLC procedure. The developed method was found distinctly rapid, precise, and eco-friendly (less solvent consuming) as compared to traditional ascending TLC.