Root endophytic microbiome dataset of sugarcane (Saccharum officinarum L.) cultivated in the Central Highlands, Vietnam, established by the 16S rRNA metagenomics

Abstract

Sugarcane (Saccharum officinarum L.) is one of the main annual crops grown in the Central Highlands of Vietnam. By understanding the taxonomic and functional profiles of root endophytic microbiome, we can develop a new cultivation technique for the sustainable production of this plant. In this work, a representative sample was obtained by mixing the roots collected from five different sugarcane fields in Dak Lak Province, the Central Highlands, in 2021. The genomic DNA of the endophytic bacteria was extracted using the DNeasy Powersoil kit, and the V1-V9 regions of the 16S rRNA genes were amplified by PCR. Libraries of 16S rRNA gene amplicons were prepared using the Swift amplicon 16S plus ITS panel kit. Finally, the Illumina MiSeq platform was used to sequence the 16S rRNA gene amplicons library. The raw data of the endophytic microbiome were uploaded to the National Center for Biotechnology Information (NCBI) with Bioproject PRJNA923851 and can be retrieved at https://www.ncbi.nlm.nih.gov/Traces/study/?acc=%20PRJNA923851. The obtained data provide basic information on the root endophytic microbiome of sugarcane cultivated in the Central Highlands of Vietnam. The data can also be useful for further developing a new technique for sustainable sugarcane production based on indigenous microorganisms. This work is the first report on the endophytic microbiome of sugarcane cultivated in this region.

Keywords: Proteobacteria; Sugarcane root endophytic microbiome dataset; biosynthesis; the 16S rRNA metagenomics.