[Effects of probenecid on the migration and proliferation ability of platelet derived growth factor-BB induced pulmonary artery smooth muscle cells in rats]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Sep;38(5):543-548. doi: 10.12047/j.cjap.6326.2022.101.
[Article in Chinese]

Abstract
in English, Chinese

Objective: To investigate whether probenecid (PROB) could improve the proliferation and migration ability of rats' pulmonary artery smooth muscle cells induced by platelet-derived growth factor-BB (PDGF-BB).

Methods: Primary pulmonary artery smooth muscle cells (PASMCs) of SD rats were cultured in vitro, and were randomly divided into control group (CON group), PDGF-BB group (10 ng/ml PDGF-BB treatment for 24 h) and PDGF-BB+PROB group (10 ng/ml PDGF-BB and 200 μmol/L PROB treatment for 24 h, PROB is a specific blocker of pannexin-1). CCK-8 method was used to select the suitable intervention concentrations of PROB and PDGF-BB, and to detect the proliferation of PASMCs in each group. The migration ability of PASMCs was detected by TranswellTM assay and cell scratch test. Immunofluorescence cytochemistry and Western blot were used to detect the protein expressions and distribution of osteopontin (OPN) and proliferating cell nuclear antigen (PCNA) in PASMCs.

Results: Compared with CON group, the migration and proliferation ability of PASMCs in PDGF-BB group were enhanced (P＜0.05). After treated with PROB, the migration and proliferation ability of PASMCs in PDGF-BB+PROB group were decreased significantly (P＜0.05). Compared with CON group, the expression and protein levels of OPN and PCNA in PDGF-BB group were increased significantly (P＜0.05), while the expression and protein levels of OPN and PCNA in PDGF-BB+PROB were decreased significantly (P＜0.05).

Conclusion: Probenecid inhibits the migration and proliferation of PDGF-BB-induced PASMCs by blocking Pannexin-1.

目的: 研究丙磺舒(PROB)对血小板衍生生长因子-BB(PDGF-BB)诱发大鼠肺动脉平滑肌细胞的迁移与增殖的影响。方法: 体外原代培养SD大鼠肺动脉平滑肌细胞(PASMCs),随机分为正常对照组(CON组)、造模组(10 ng/ml PDGF-BB 24 h)、丙磺舒处理组(10 ng/ml PDGF-BB和200 μmol/L PROB孵育24 h,PROB为Pannexin-1的特异性阻断剂)。通过CCK-8法选取丙磺舒和PDGF-BB干预浓度,并检测各组细胞的增殖能力;通过细胞划痕实验和TranswellTM实验检测各组PASMCs的迁移能力;通过细胞免疫荧光技术检测各组PASMCs中骨桥蛋白(OPN)和增殖细胞核抗原(PCNA)的分布和表达;通过Western blot法检测各组PASMCs中OPN和PCNA的蛋白水平差异。结果: 与CON组相比,PDGF-BB组的PASMCs迁移和增殖能力均有所增强(P＜0.05),经PROB干预后,细胞迁移和增殖能力降低(P＜0.05);与CON组相比,PDGF-BB组OPN和PCNA的表达和蛋白水平均显著升高(P＜0.05),经PROB干预后,其表达和蛋白水平显著降低(P＜0.05)。结论: 丙磺舒通过阻断Pannexin-1可以抑制PDGF-BB诱发PASMCs的增殖和迁移。.

Keywords: PASMCs; migration; pannexin-1; probenecid; proliferation.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Becaplermin / metabolism
  • Becaplermin / pharmacology
  • Cell Proliferation
  • Cells, Cultured
  • Myocytes, Smooth Muscle
  • Probenecid* / metabolism
  • Probenecid* / pharmacology
  • Proliferating Cell Nuclear Antigen / metabolism
  • Proto-Oncogene Proteins c-sis / metabolism
  • Proto-Oncogene Proteins c-sis / pharmacology
  • Pulmonary Artery*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Becaplermin
  • Proliferating Cell Nuclear Antigen
  • Probenecid
  • Proto-Oncogene Proteins c-sis