Effects of Chinese fir planting and phosphorus addition on soil microbial biomass and extracellular enzyme activities

Meng-Ke Dou; Wei-Dong Zhang; Qing-Peng Yang; Long-Chi Chen; Ye-Jia Liu; Ya-Lin Hu

doi:10.13287/j.1001-9332.202303.003

Effects of Chinese fir planting and phosphorus addition on soil microbial biomass and extracellular enzyme activities

Ying Yong Sheng Tai Xue Bao. 2023 Mar;34(3):631-638. doi: 10.13287/j.1001-9332.202303.003.

Authors

Meng-Ke Dou¹, Wei-Dong Zhang^{2

3}, Qing-Peng Yang^{2

3}, Long-Chi Chen^{2

3}, Ye-Jia Liu⁴, Ya-Lin Hu¹

Affiliations

¹ Forest Ecology & Stable Isotope Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
² Institute of Applied Ecology, Chinese Academy of Sciences/Key Laboratory of Forest Ecology and Management, Chinese Academy of Sciences/Huitong Experimental Station of Forest Ecology, Shenyang 110016, China.
³ Hunan Key Laboratory for Structure and Ecosystem Service of Subtropical Forest, Huitong 418307, Hunan, China.
⁴ Forestry Department of Yuanling County, Yuanling 419699, Hunan, China.

PMID: 37087645
DOI: 10.13287/j.1001-9332.202303.003

Abstract
in English, Chinese

Plants can alter soil microbial biomass and extracellular enzyme activities related with carbon (C), nitrogen (N), and phosphorus (P), through litter and root exudates, with consequences on soil carbon, nitrogen and phosphorus (P) cycling. However, it is not well known how the changes in soil phosphorus availability affect the relationships between plants and soil microorganisms. In this study, a factorial experiment was conducted to investigate the effects of Chinese fir (Cunninghamia lanceolata) planting and different levels of P addition (0, 1.95, 3.9, 7.8 and 15.6 g P·m^-2·a^-1) on soil microbial biomass and extracellular enzyme activities. The results showed that planting Chinese fir planting significantly altered soil microbial biomass and C- and N- and P-related extracellular enzyme activities, but the effects were dependent on P addition levels. Without P addition, Chinese fir planting significantly reduced soil nutrient availability and pH, which led to the aggravation of P limitation and lower soil microbial biomass. P addition relieved P limitation, and reduced soil acid phosphatase (ACP) activities by 30.0%, 30.5%, 35.3% and 47.1% with the increasing P addition level (1.95, 3.9, 7.8 and 15.6 g P·m^-2·a^-1). Under three P addition levels (1.95, 3.9 and 7.8 g P·m^-2·a^-1), the negative effects of Chinese fir planting on soil microbial growth were alleviated. Under the high P addition level (15.6 g P·m^-2·a^-1), the negative effects of Chinese fir planting on soil microbial growth occurred again due to soil N limitation. Taken together, Chinese fir planting and soil P availability generally affected soil microbial biomass and extracellular enzyme activities, and changed P limitation.

植物可以通过凋落物和根系分泌物改变土壤微生物生物量及碳氮磷转化胞外酶活性,进而影响土壤碳氮磷循环过程。然而,针对土壤磷有效性如何影响植物与土壤微生物之间的关系依然不清楚。本研究在不同磷添加水平下(0、1.95、3.9、7.8、15.6 g P·m^-2·a^-1),分析杉木种植对土壤微生物生物量和碳氮磷转化胞外酶活性的影响。结果表明: 杉木种植显著改变了土壤微生物生物量与碳氮磷转化胞外酶活性,而且影响程度受磷添加水平的调控。在不添加磷处理下,种植杉木显著降低了土壤养分有效性,并引起土壤酸化,导致磷限制加剧,抑制土壤微生物生物量。磷添加逐渐减缓磷限制,随着磷添加水平的升高(1.95、3.9、7.8、15.6 g P·m^-2·a^-1)土壤酸性磷酸酶活性比对照分别降低30.0%、30.5%、35.3%和47.1%。在3个磷添加水平处理下(1.95、3.9、7.8 g P·m^-2·a^-1),杉木对土壤微生物生长的抑制作用得到缓解。在高水平磷添加处理下(15.6 g P·m^-2·a^-1),由于磷添加引起的氮养分限制,杉木种植对土壤微生物再次呈抑制作用。杉木种植和土壤磷有效性共同调控土壤微生物生物量与胞外酶活性,改变磷限制。.

Keywords: Chinese fir planting; extracellular enzyme activity; nutrient limitation; phosphorus addition; soil microbial biomass.

MeSH terms

Biomass
Carbon
Cunninghamia*
Nitrogen / analysis
Phosphorus
Soil / chemistry
Soil Microbiology

Substances

Soil
Phosphorus
Carbon
Nitrogen

Abstract in English, Chinese

MeSH terms

Substances

Abstract
in English, Chinese