Effects of various thermal treatments on interfacial composition and physical properties of bovine milk fat globules

Abstract

This study aimed to investigate changes of milk fat globules (MFG) and their membranes after thermal treatments, and further analyzed the relationship between the stability of MFG and interfacial compositions of milk fat globule membrane (MFGM). We characterized the influence of three kinds of thermal treatments on fat globule interfacial components (including interfacial phospholipids and interfacial protein) and physical properties using phospholipidomics and several microscopy techniques. The results showed that size of MFG increased from 2.96 μm to 3.59 μm and ζ-potential decreased from -9.71 mV to -13.23 mV after thermal treatment, suggesting that MFGM was damaged and MFG occurred coalescence. Thermal treatment increased the Young's modulus of MFGM and made membranes more fragile. The abundance of MFGM proteins decreased while casein and β-lactoglobulin increased after thermal treatment. Results of phospholipidomics showed that 27 phospholipid species could be used to distinguish the samples. Pasteurization reduced mainly SM and PC located in the outer bilayer of MFGM, while ultra-pasteurization reduced not only SM and PC but also PI and PE located in the inner leaflet. Based on correlation analysis, the increase in Young's modulus of MFGM during thermal treatment might be related to changes in chemical components on the membrane, suggesting a potential link between the change of MFGM components and fat globule coalescence behavior.

Keywords: Cow milk; Fat globule coalescence; Milk fat globule membrane; Phospholipid; Protein; The Young’s modulus.