Rapid discrimination of Brucellosis in sheep using serum Fourier transform infrared spectroscopy combined with PCA-LDA algorithm

Jingrui Dou; Wubulitalifu Dawuti; Xiangxiang Zheng; Yousen Zhu; Renyong Lin; Guodong Lü; Yujiang Zhang

doi:10.1016/j.pdpdt.2023.103567

Rapid discrimination of Brucellosis in sheep using serum Fourier transform infrared spectroscopy combined with PCA-LDA algorithm

Photodiagnosis Photodyn Ther. 2023 Jun:42:103567. doi: 10.1016/j.pdpdt.2023.103567. Epub 2023 Apr 20.

Authors

Jingrui Dou¹, Wubulitalifu Dawuti¹, Xiangxiang Zheng², Yousen Zhu³, Renyong Lin⁴, Guodong Lü⁵, Yujiang Zhang⁶

Affiliations

¹ School of Public Health, Xinjiang Medical University, Urumqi 830054, China; State Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
² School of Electronic Engineering, Beijing University of Posts and Telecommunications, Beijing 100876, China.
³ Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
⁴ State Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
⁵ School of Public Health, Xinjiang Medical University, Urumqi 830054, China; State Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China. Electronic address: lgd_xj@qq.com.
⁶ School of Public Health, Xinjiang Medical University, Urumqi 830054, China; The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi 830002, China. Electronic address: xjsyZhang@163.com.

PMID: 37084931
DOI: 10.1016/j.pdpdt.2023.103567

Abstract

Brucellosis in sheep is an infectious disease caused by Brucella melitensis in sheep. The current conventional serological methods for screening Brucella-infected sheep have the disadvantage of time consuming and low accuracy, so a simple, rapid and highly accurate screening method is needed. The aim of this study was to evaluate the feasibility of diagnosing Brucella-infected sheep by serum samples based on the Fourier transform infrared (FTIR) spectroscopy. In this study, FTIR spectroscopy of serum from Brucella-infected sheep (n = 102) and healthy sheep (n = 125) revealed abnormal protein and lipid metabolism in serum from Brucella-infected sheep compared to healthy sheep. Principal component analysis-Linear discriminant analysis (PCA-LDA) method was used to differentiate the FTIR spectra of serum from Brucella-infected sheep and healthy sheep in the protein band (3700-3090 cm^-1) and lipid band (3000-2800 cm^-1), and its overall diagnostic accuracy was 100% (sensitivity 100%, specificity 100%). In conclusion, our results suggest that serum FTIR spectroscopy combined with PCA-LDA algorithm has great potential for brucellosis in sheep screening.

Keywords: Brucellosis; FTIR spectroscopy; Principal component analysis-Linear discriminant analysis (PCA-LDA); Serum; Sheep.

MeSH terms

Animals
Brucellosis* / diagnosis
Brucellosis* / veterinary
Discriminant Analysis
Photochemotherapy* / methods
Photosensitizing Agents
Principal Component Analysis
Sheep
Sheep Diseases* / diagnosis
Spectroscopy, Fourier Transform Infrared / methods

Substances

Photosensitizing Agents