Single-cell transcriptomics of a dynamic cell behavior in murine airways

Sheldon J J Kwok; Daniel T Montoro; Adam L Haber; Seok-Hyun Yun; Vladimir Vinarsky

doi:10.7554/eLife.76645

Single-cell transcriptomics of a dynamic cell behavior in murine airways

Elife. 2023 Apr 21:12:e76645. doi: 10.7554/eLife.76645.

Authors

Sheldon J J Kwok^#^{1

2}, Daniel T Montoro^#^{3

4}, Adam L Haber^#⁵, Seok-Hyun Yun^{1

2}, Vladimir Vinarsky^{6

7}

Affiliations

¹ Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Cambridge, United States.
² Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, United States.
³ Broad Institute of MIT and Harvard, Cambridge, United States.
⁴ Department of Systems Biology, Harvard Medical Schoo, Boston, United States.
⁵ Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, United States.
⁶ Center for Regenerative Medicine, Massachusetts General Hospital, Boston, United States.
⁷ Division of Pulmonary and Critical Care Medicine, Department of Medicine, Massachusetts General Hospital, Boston, United States.

^# Contributed equally.

Abstract

Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple the physiological behaviors of cells in an intact murine tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following injury in murine airways.

Keywords: airways; developmental biology; migration; mouse; regenerative medicine; stem cells; two-photon imaging.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Gene Expression Profiling*
Mice
Single-Cell Analysis / methods
Transcriptome*

Associated data

GEO/GSE193954
GEO/GSE102580

Abstract

Publication types

MeSH terms

Associated data

Grants and funding