Hydrogen peroxide treatment mitigates antibiotic resistance gene and mobile genetic element propagation in mariculture sediment

Ying Han; Haodong Wang; Jiayue Wu; Yikai Hu; Hexin Wen; Zijian Yang; Hao Wu

doi:10.1016/j.envpol.2023.121652

Hydrogen peroxide treatment mitigates antibiotic resistance gene and mobile genetic element propagation in mariculture sediment

Environ Pollut. 2023 Jul 1:328:121652. doi: 10.1016/j.envpol.2023.121652. Epub 2023 Apr 19.

Authors

Ying Han¹, Haodong Wang², Jiayue Wu², Yikai Hu², Hexin Wen², Zijian Yang², Hao Wu³

Affiliations

¹ School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China; Hebei Province Key Laboratory of Deep Remediation of Heavy Metals in Water and Resource Utilization, Yanshan University, Qinhuangdao, 066004, PR China. Electronic address: hanying@ysu.edu.cn.
² School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China.
³ School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China; Hebei Province Key Laboratory of Deep Remediation of Heavy Metals in Water and Resource Utilization, Yanshan University, Qinhuangdao, 066004, PR China.

PMID: 37080523
DOI: 10.1016/j.envpol.2023.121652

Abstract

Mariculture sediments have been exchange and propagation sources of antibiotic resistance genes (ARGs). However, no efficient methods have been generated to remove ARGs from sediments. Here, we explored the impact of hydrogen peroxide (H₂O₂) and aeration on the efficient removal of ARGs and mobile genetic elements (MGEs) in mariculture sediments. When compared with the aeration group, the ARG abundance was 3.8-32.3% lower in the H₂O₂ group during the first 14 days. ARG and MGE abundances were also significantly associated with reduced total bacterial population and diversity (P < 0.05). Based on partial squares path modeling, reduction of MGEs had important roles in ARG removal from H₂O₂ treatments, while in the aeration group, ARG reductions were mainly determined by changes in bacterial community composition. These results suggested that H₂O₂ treatment represent a promising method for controlling ARG abundance after dosing feed stuff and limit the spread of ARGs in aquaculture environments.

Keywords: Antibiotic resistance genes; Bacterial communities; Hydrogen peroxide treatment; Mariculture sediment; Mobile genetic elements.

MeSH terms

Anti-Bacterial Agents* / pharmacology
Bacteria / genetics
Drug Resistance, Microbial / genetics
Genes, Bacterial*
Hydrogen Peroxide / pharmacology
Interspersed Repetitive Sequences

Substances

Anti-Bacterial Agents
Hydrogen Peroxide