Enhanced phytoremediation of petroleum-contaminated soil by biochar and urea

Abstract

Application of bioremediation in petroleum-contaminated soils is limited by its low efficiency. Although biochar and urea are commonly used soil additives, their potential beneficial effect on the bioremediation of petroleum contamination have rarely been discussed. In this study, biochar and urea were combined to test their effects on the phytoremediation of petroleum-contaminated soil in pot experiments. Our results showed that the degradation rate of total petroleum hydrocarbons reached 49.6%, 38.3%, 42.5%, and 77.9% when the soil was treated with biochar, urea, ryegrass, and their integrated application treatment (PBCN), respectively. A number of soil physicochemical properties (e.g., pH, elements, aggregate distribution, and organic matter composition) altered by the treatments were found to be linked to the accelerated degradation of petroleum hydrocarbons. The activities of soil dehydrogenase, lipase, and urease, and the abundances of 16 s rRNA gene and alkane degradation-related genes could be increased simultaneously when biochar, urea, and ryegrass were co-applied. Furthermore, urea significantly reduced soil bacterial α-diversity, while soil bacterial community dissimilation was mainly driven by urea and ryegrass. Lysobacter, xanthomonadaceae, and longimicrobia could be biomarker species in the PBCN group. Soil bacterial network analysis showed that biochar and urea application decreased the network complexity and robustness, while ryegrass behaved inversely. Lastly, soil metabolomic analysis revealed that root soil metabolites were greatly affected by urea-addition during phytoremediation, and co-application of biochar and urea could activate the putative metabolism pathway of petroleum hydrocarbons in root soil (e.g., naphthalene and anthracene degradation, and pyruvate metabolism). In summary, this study confirmed the enhancement of biochar and urea application in the phytoremediation of petroleum-contaminated soil and explored the internal mechanism of the interactive effect, which can potentially improve the development of eco-friendly and cost-effective in-situ bioremediation technology for petroleum-contaminated soils.

Keywords: Biochar amendment; Petroleum-contaminated soil; Phytoremediation; Soil bacterial community; Soil metabolome.