3D stimulated Raman spectral imaging of water dynamics associated with pectin-glycocalyceal entanglement

Moritz Floess; Tobias Steinle; Florian Werner; Yunshan Wang; Willi L Wagner; Verena Steinle; Betty S Liu; Yifan Zheng; Zi Chen; Maximilian Ackermann; Steven J Mentzer; Harald Giessen

doi:10.1364/BOE.485314

3D stimulated Raman spectral imaging of water dynamics associated with pectin-glycocalyceal entanglement

Biomed Opt Express. 2023 Mar 7;14(4):1460-1471. doi: 10.1364/BOE.485314. eCollection 2023 Apr 1.

Authors

Moritz Floess¹, Tobias Steinle¹, Florian Werner¹, Yunshan Wang¹, Willi L Wagner^{2

3}, Verena Steinle², Betty S Liu⁴, Yifan Zheng⁴, Zi Chen⁴, Maximilian Ackermann^{5

6}, Steven J Mentzer⁴, Harald Giessen¹

Affiliations

¹ 4 Physics Institute and Stuttgart Research Center of Photonic Engineering, University of Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany.
² Department of Diagnostic and Interventional Radiology, University Hospital of Heidelberg, Im Neuenheimer Feld 420, 69120 Heidelberg, Germany.
³ Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), University of Heidelberg, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany.
⁴ Laboratory of Adaptive and Regenerative Biology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
⁵ Institute of Pathology and Department of Molecular Pathology, Helios University Clinic Wuppertal, University of Witten-Herdecke, Wuppertal, Germany.
⁶ Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.

Abstract

Pectin is a heteropolysaccharide responsible for the structural integrity of the cell walls of terrestrial plants. When applied to the surface of mammalian visceral organs, pectin films form a strong physical bond with the surface glycocalyx. A potential mechanism of pectin adhesion to the glycocalyx is the water-dependent entanglement of pectin polysaccharide chains with the glycocalyx. A better understanding of such fundamental mechanisms regarding the water transport dynamics in pectin hydrogels is of importance for medical applications, e.g., surgical wound sealing. We report on the water transport dynamics in hydrating glass-phase pectin films with particular emphasis on the water content at the pectin-glycocalyceal interface. We used label-free 3D stimulated Raman scattering (SRS) spectral imaging to provide insights into the pectin-tissue adhesive interface without the confounding effects of sample fixation, dehydration, shrinkage, or staining.

Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.