Novel and Efficient Method for Diagnosing Infective Endocarditis Using 16S Ribosomal RNA Gene Amplicon Sequence

Akihisa Kataoka; Takahiko Shiba; Sayaka Katagiri; Shuhei Koyama; Kento Kito; Tatsuya Ota; Taiga Katayama; Yujin Ohsugi; Takahiko Nagai; Ryota Kobayashi; Keiji Komatsu; Masahiro Urata; Tomohiro Imazuru; Takanori Iwata; Ken Kozuma

doi:10.1016/j.jaccas.2023.101793

Novel and Efficient Method for Diagnosing Infective Endocarditis Using 16S Ribosomal RNA Gene Amplicon Sequence

JACC Case Rep. 2023 Feb 22:11:101793. doi: 10.1016/j.jaccas.2023.101793. eCollection 2023 Apr 5.

Authors

Affiliations

¹ Department of Medicine, Division of Cardiology, Teikyo University, Tokyo, Japan.
² Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
³ Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA.
⁴ Department of Lifetime Oral Health Care Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
⁵ Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan.

Abstract

We present 2 cases that both developed infective endocarditis and underwent mitral valve replacement. In addition to positive blood culture and echocardiographic findings, such as vegetation or mitral valve perforation, the 16S ribosomal RNA gene amplicon sequence approach used was helpful for disease diagnosis. (Level of Difficulty: Intermediate.).

Keywords: echocardiography; endocarditis; genetics; mitral valve.

Publication types

Case Reports