Imaging Brown Adipose Tissue with TSPO PET Tracers in Preclinical Animal Studies

Biyue Zhu; Steven H Liang; Chongzhao Ran

doi:10.1007/978-1-0716-3167-6_13

Imaging Brown Adipose Tissue with TSPO PET Tracers in Preclinical Animal Studies

Methods Mol Biol. 2023:2662:147-156. doi: 10.1007/978-1-0716-3167-6_13.

Authors

Biyue Zhu¹, Steven H Liang², Chongzhao Ran³

Affiliations

¹ Molecular Imaging Laboratory, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA.
² Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
³ Molecular Imaging Laboratory, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA. CRAN@mgh.harvard.edu.

PMID: 37076678
DOI: 10.1007/978-1-0716-3167-6_13

Abstract

Brown adipose tissue (BAT) is closely associated with thermogenesis and related to numerous diseases, including type 2 diabetes, nonalcoholic fatty liver disease (NAFLD), and obesity. Using molecular imaging technologies to monitor BAT could facilitate etiology elucidation, disease diagnosis, and therapeutics development. Translocator protein (TSPO), an 18 kDa protein that mainly locates on the outer mitochondrial membrane, has been proven as a promising biomarker for monitoring BAT mass. Here, we lay out the steps for imaging BAT with TSPO PET tracer [¹⁸F]-DPA in mouse studies.

Keywords: Brown adipose tissue (BAT); Positron emission tomography (PET); Translocator protein (TSPO).

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adipose Tissue, Brown* / diagnostic imaging
Adipose Tissue, Brown* / metabolism
Animals
Animals, Laboratory
Carrier Proteins / metabolism
Diabetes Mellitus, Type 2* / metabolism
Mice
Positron-Emission Tomography / methods

Substances

Carrier Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding