Disto-TRP: An approach for identifying transient receptor potential (TRP) channels using structural information generated by AlphaFold

Abstract

The ability to predict 3D protein structures computationally has significantly advanced biological research. The AlphaFold protein structure database, developed by DeepMind, has provided a wealth of predicted protein structures and has the potential to bring about revolutionary changes in the field of life sciences. However, directly determining the function of proteins from their structures remains a challenging task. The Distogram from AlphaFold is used in this study as a novel feature set to identify transient receptor potential (TRP) channels. Distograms feature vectors and pre-trained language model (BERT) features were combined to improve prediction performance for transient receptor potential (TRP) channels. The method proposed in this study demonstrated promising performance on many evaluation metrics. For five-fold cross-validation, the method achieved a Sensitivity (SN) of 87.00%, Specificity (SP) of 93.61%, Accuracy (ACC) of 93.39%, and a Matthews correlation coefficient (MCC) of 0.52. Additionally, on an independent dataset, the method obtained 100.00% SN, 95.54% SP, 95.73% ACC, and an MCC of 0.69. The results demonstrate the potential for using structural information to predict protein function. In the future, it is hoped that such structural information will be incorporated into artificial intelligence networks to explore more useful and valuable functional information in the biological field.

Keywords: AlphaFold; Distance map; Distogram; Protein structure; TRP channels.