Protein dynamics by the combination of high-speed AFM and computational modeling

Holger Flechsig; Toshio Ando

doi:10.1016/j.sbi.2023.102591

Protein dynamics by the combination of high-speed AFM and computational modeling

Curr Opin Struct Biol. 2023 Jun:80:102591. doi: 10.1016/j.sbi.2023.102591. Epub 2023 Apr 17.

Authors

Holger Flechsig¹, Toshio Ando²

Affiliations

¹ Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
² Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan. Electronic address: tando@staff.kanazawa-u.ac.jp.

PMID: 37075535
DOI: 10.1016/j.sbi.2023.102591

Abstract

High-speed atomic force microscopy (HS-AFM) allows direct observation of biological molecules in dynamic action. However, HS-AFM has no atomic resolution. This article reviews recent progress of computational methods to infer high-resolution information, including the construction of 3D atomistic structures, from experimentally acquired resolution-limited HS-AFM images.

Publication types

Review

MeSH terms

Computer Simulation*
Microscopy, Atomic Force / methods