Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma

Allie Heller; Fang Du; Yongqiang Liu; Yijun Yang; Zeng-Jie Yang

doi:10.1016/j.xpro.2023.102234

Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma

STAR Protoc. 2023 Apr 18;4(2):102234. doi: 10.1016/j.xpro.2023.102234. Online ahead of print.

Authors

Allie Heller¹, Fang Du¹, Yongqiang Liu¹, Yijun Yang², Zeng-Jie Yang³

Affiliations

¹ Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA.
² Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA. Electronic address: yijun.yang@fccc.edu.
³ Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA. Electronic address: zengjie.yang@fccc.edu.

Abstract

Tumor relapse is the leading adverse prognostic factor in medulloblastoma (MB). However, there is still no established mouse model for MB relapse, impeding our efforts to develop strategies to treat relapsed MB. We present a protocol for generating a mouse model for relapsed MB using irradiation by optimizing mouse breeding and age, as well as irradiation dosage and timing. We then detail procedures for determining tumor relapse based on tumor cell trans-differentiation in MB tissue, immunohistochemistry, and tumor cell isolation. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).¹.

Keywords: Cancer; Cell Isolation; Microscopy; Model Organisms; Neuroscience.