Development of Spectral Imaging Cytometry

Methods Mol Biol. 2023:2635:3-22. doi: 10.1007/978-1-0716-3020-4_1.

Abstract

Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.

Keywords: Aurora cytometer; Autofluorescence; Flow cytometry; Fluorescence spectra; Sony spectral analyzer; Spectral cytometry; Spectral unmixing; Virtual filtering.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diagnostic Imaging*
  • Flow Cytometry / methods
  • Fluorescent Dyes*
  • Mammals

Substances

  • Fluorescent Dyes