The pyrin inflammasome detects bacterial toxins and effectors that inhibit RhoA GTPases and triggers inflammatory cytokine release and a fast cell death termed pyroptosis. In addition, various endogenous molecules, drugs, synthetic molecules, or mutations can trigger pyrin inflammasome activation. The pyrin protein differs between humans and mice, and the repertoire of pyrin activators is also species-specific. Here, we present the various pyrin inflammasome activators, inhibitors, the kinetics of pyrin activation in response to the various activators, and their species specificity. In addition, we present different methods to monitor pyrin-mediated pyroptosis.
Keywords: Colchicine; IL-1 β; MEFV; Pregnanolone; Real-time cell death assay; Statin; TcdA; TcdB; Toxin.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.