Dual-slit confocal light-sheet microscopy using birefringent crystals

Xin Xu; Hong Ye; Jixiang Wang; Xin Zhang; Guohua Shi

doi:10.1002/jbio.202300050

Dual-slit confocal light-sheet microscopy using birefringent crystals

J Biophotonics. 2023 Jul;16(7):e202300050. doi: 10.1002/jbio.202300050. Epub 2023 Apr 27.

Authors

Xin Xu^{1

2}, Hong Ye², Jixiang Wang^{1

2}, Xin Zhang², Guohua Shi^{1

2}

Affiliations

¹ School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou, China.

PMID: 37070639
DOI: 10.1002/jbio.202300050

Abstract

We present a method to use a birefringent crystal for generating two illumination beams in a digital scanned laser light-sheet microscopy (DSLM) system. Upon this, a conventional confocal DSLM can be easily upgraded to a dual-slit confocal DSLM with two-fold imaging speed. We have implemented this method to our bidirectional DSLM system, locating two identical calcite crystals on both illumination paths from both sides of the sample. The neurons of in vivo larval zebrafish have been fast imaged with sterling image quality, especially ~2.5 times higher contrast, compared to the conventional DSLM.

Keywords: confocal; dual-slit; high-speed; light-sheet microscope.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Birefringence
Larva
Microscopy, Confocal / methods
Neurons*
Zebrafish*