Phylogenetic analysis of 34 monkeypox virus genome sequences isolated from patients in Minas Gerais, Brazil, revealed initial importation events in early June 2022, then community transmission within the state. All generated genomes belonged to the B.1 lineage responsible for a global mpox outbreak. These findings can inform public health measures.
Keywords: Brazil; Minas Gerais; Monkeypox virus; genomic surveillance; mpox; sexually transmitted diseases; viruses; zoonoses.