A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3

Front Cell Infect Microbiol. 2023 Mar 28:13:1177866. doi: 10.3389/fcimb.2023.1177866. eCollection 2023.

Abstract

Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.

Keywords: CRISPR/Cas9; DAdV-3; expression; fiber-2; recombinant FAdV-4; replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae Infections* / pathology
  • Animals
  • Antibodies, Viral
  • Aviadenovirus* / genetics
  • Chickens
  • Ducks
  • Poultry Diseases*
  • Serogroup
  • Vaccines*

Substances

  • Antibodies, Viral
  • Vaccines

Grants and funding

This study was supported by the Science and Innovation Program for college students (202111117014), the 111 Project (D18007), Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality (26116120), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.