The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method

Yirong Wang; Jingchun Chen; Jinpan Du; Liming Lei; Boxin Zhao; Yunpeng Bai; Dong Chen; Xipei Wang; Chunbo Chen

doi:10.1155/2023/6674009

The Determination of Polymyxin B in Critically Ill Patients by the HPLC-MS/MS Method

Int J Anal Chem. 2023 Apr 7:2023:6674009. doi: 10.1155/2023/6674009. eCollection 2023.

Authors

Yirong Wang¹, Jingchun Chen¹, Jinpan Du¹, Liming Lei², Boxin Zhao³, Yunpeng Bai⁴, Dong Chen⁵, Xipei Wang^{6

7}, Chunbo Chen^{1

2

8

9}

Affiliations

¹ School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
² Department of Intensive Care Unit of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
³ Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, China.
⁴ Center of Scientific Research, Maoming People's Hospital, Maoming 525000, China.
⁵ School of Medicine, South China University of Technology, Guangzhou 510006, China.
⁶ Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.
⁷ Guangdong Provincial Key Laboratory of Clinical Pharmacology, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.
⁸ Department of Critical Care Medicine, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of South University of Science and Technology, Shenzhen 518020, China.
⁹ Department of Emergency, Maoming People's Hospital, Maoming 525000, China.

Abstract

Polymyxin B (PB) is a dose-dependent drug used to treat multidrug-resistantgram-negative bacteria, for which a suitable method is needed to determine clinical samples. A simple, economical, and efficient high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method was developed and validated for polymyxin B1 (PB1), polymyxin B1-Ile (PB1-I), polymyxin B2 (PB2), and polymyxin B3 (PB3) in human plasma. Chromatographic column was Waters BEH C18 column (2.1 × 50 mm, 1.7 μm). Phase A was water with 0.2% formic acid (FA), and phase B was acetonitrile containing 0.2% FA. The elution method is gradient elutio. The total analysis time was 5 min. The pretreatment method involved protein precipitation using acetonitrile containing 0.2% trifluoroacetic acid and 0.1% FA as the precipitant. The recovery rate was 92-99%. The total quantity of PB1 and PB1-I was measured in the linear range of 100-8000 ng/mL. Simultaneously, the total amounts of PB2 and PB3 were measured in the linear range of 11.9-948.5 ng/mL. This validated method was successfully applied to the pharmacokinetics of PB in critically ill patients.