Identification of two cassava receptor-like cytoplasmic kinase genes related to disease resistance via genome-wide and functional analysis

Yiwei Ye; Zhiwei Ouyang; Changlin Guo; Yanna Wu; Jianjun Li; Assane Hamidou Abdoulaye; Li Tang; Wei Xia; Yinhua Chen

doi:10.1016/j.ygeno.2023.110626

Identification of two cassava receptor-like cytoplasmic kinase genes related to disease resistance via genome-wide and functional analysis

Genomics. 2023 May;115(3):110626. doi: 10.1016/j.ygeno.2023.110626. Epub 2023 Apr 14.

Authors

Yiwei Ye¹, Zhiwei Ouyang², Changlin Guo¹, Yanna Wu¹, Jianjun Li¹, Assane Hamidou Abdoulaye¹, Li Tang³, Wei Xia¹, Yinhua Chen⁴

Affiliations

¹ College of Tropical Crops, Hainan University, Haikou, China.
² HNU-ASU Joint International Tourism College, Hainan University, Haikou, China.
³ Agricultural Environment and Soil Research Institute, Hainan Academy of Agricultural Sciences, China.
⁴ Sanya Nanfan Research Institute of Hainan University, Sanya 572025, China. Electronic address: yhchen@hainanu.edu.cn.

PMID: 37062363
DOI: 10.1016/j.ygeno.2023.110626

Abstract

Receptor-like cytoplasmic kinases (RLCKs) play important roles in various developmental processes and stress responses in plants. Whereas, the detailed information of this family in cassava has not clear yet. In this study, A total of 322 MeRLCK genes were identified in the cassava genome, and they could be divided into twelve clades (Clades I-XII) according to their phylogenetic relationships. Most RLCK members in the same clade have similar characteristics and motif compositions. Over half of the RLCKs possess cis-elements in their promoters that respond to ABA, MeJA, defense reactions, and stress. Under Xpm11 infection, the expression levels of four genes show significant changes, suggesting their involvement in Xpm11 resistance. Two RLCK (MeRLCK11 and MeRLCK84) genes potentially involved in resistance to cassava bacterial blight were identified through VIGS experiments. This work laid the foundation for studying the function of the cassava RLCK genes, especially the genes related to pathogen resistance.

Keywords: Cassava; Cassava bacterial blight; Disease resistance; RLCK gene family; Virus-induced gene silencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance
Gene Expression Regulation, Plant
Manihot* / genetics
Manihot* / metabolism
Manihot* / microbiology
Phylogeny
Plant Proteins / genetics

Substances

Plant Proteins