Employing salt-tolerant bacteria Serratia marcescens subsp. SLS for biodegradation of oily kitchen waste

Xia Zhang; Dan Zhang; Shaohua Chu; Muhammad Khalid; Renyuan Wang; Yaowei Chi; Xiangyu Duan; Xijia Yang; Pei Zhou

doi:10.1016/j.chemosphere.2023.138655

Employing salt-tolerant bacteria Serratia marcescens subsp. SLS for biodegradation of oily kitchen waste

Chemosphere. 2023 Jul:329:138655. doi: 10.1016/j.chemosphere.2023.138655. Epub 2023 Apr 12.

Authors

Xia Zhang¹, Dan Zhang², Shaohua Chu², Muhammad Khalid³, Renyuan Wang¹, Yaowei Chi¹, Xiangyu Duan¹, Xijia Yang², Pei Zhou⁴

Affiliations

¹ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, 200240, China.
² School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, 200240, China; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, Shanghai, 200240, China; Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, China.
³ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
⁴ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, 200240, China; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, Shanghai, 200240, China; Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: peizhousjtu@163.com.

PMID: 37059197
DOI: 10.1016/j.chemosphere.2023.138655

Abstract

The high oil and salt content of kitchen waste (KW) inhibit bioconversion and humus production. To efficiently degrade oily kitchen waste (OKW), a halotolerant bacterial strain, Serratia marcescens subsp. SLS which could transform various animal fats and vegetable oils, was isolated from KW compost. Its identification, phylogenetic analysis, lipase activity assays, and oil degradation in liquid medium were assessed, and then it was employed to carry out a simulated OKW composting experiment. In liquid medium, the 24 h degradation rate of mixed oils (soybean oil: peanut oil: olive oil: lard = 1:1:1:1, v/v/v/v) was up to 87.37% at 30 °C, pH 7.0, 280 rpm, 2% oil concentration and 3% NaCl concentration. The ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS) method demonstrated that the mechanism of SLS strain metabolizing long-chain triglycerides (TAGs) (C53-C60), especially the biodegradation of TAG (C18:3/C18:3/C18:3) by the strain can reach more than 90%. Degradation of 5, 10, 15% concentrations of total mixed oil were also calculated to be 64.57, 71.25, 67.99% respectively after a simulated composting duration of 15 days. The results suggest that the isolated strain of S. marcescens subsp. SLS is suitable for OKW bioremediation in high NaCl concentration within a reasonably short period of time. The findings introduced a salt-tolerant and oil-degrading bacteria, providing insights into the mechanism of oil biodegradation and offering new avenues of study for OKW compost and oily wastewater treatment.

Keywords: Biodegradation; Composting; High NaCl concentration; Oil-degrading; Oily kitchen waste; Serratia marcescens subsp. SLS.

MeSH terms

Biodegradation, Environmental
Chromatography, Liquid
Phylogeny
Plant Oils
Serratia marcescens* / metabolism
Sodium Chloride* / metabolism
Tandem Mass Spectrometry

Substances

Sodium Chloride
Plant Oils