Generation of an A549 ISRE-luciferase stable cell line

Laurie-Anne Lamotte; Lionel Tafforeau

doi:10.1016/j.jviromet.2023.114731

Generation of an A549 ISRE-luciferase stable cell line

J Virol Methods. 2023 Jun:316:114731. doi: 10.1016/j.jviromet.2023.114731. Epub 2023 Apr 13.

Authors

Laurie-Anne Lamotte¹, Lionel Tafforeau²

Affiliations

¹ Cell Biology laboratory, Research Institute for Biosciences, Research Institute for Health Sciences and Technology, University of Mons, 7000 Mons, Belgium.
² Cell Biology laboratory, Research Institute for Biosciences, Research Institute for Health Sciences and Technology, University of Mons, 7000 Mons, Belgium. Electronic address: lionel.tafforeau@umons.ac.be.

PMID: 37059128
DOI: 10.1016/j.jviromet.2023.114731

Abstract

With its human lung origin, A549 cell line is a designated cellular model for viral respiratory infections studies. As such infections are known to lead to innate immune responses, various IFN signaling modifications occur in infected cells and have to be considered in respiratory viruses experiments. Here, we describe the generation of an A549 stable cell line that expresses firefly luciferase upon interferon-β stimulation, as well as upon RIG-I transfection and upon influenza A virus infection. Of the 18 clones generated, the first one, namely A549-RING1, demonstrated appropriate luciferase expression in the different conditions tested. This newly established cell line may therefore be used to decipher the impact of viral respiratory infection on innate immune response depending on IFN stimulation, without any plasmid transfection step. A549-RING1 can be provided upon request.

Keywords: IFN-β; ISRE-Luciferase; Influenza A virus; Innate immune response; RIG-I; Viral infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Humans
Immunity, Innate
Influenza A virus*
Influenza, Human*
Luciferases / genetics

Substances

Luciferases