Objective: To investigate the role of chemokine receptor ACKR2 in cognitive impairment in young rats with traumatic brain injury.
Methods: Seventy-five young rats were randomly divided into TBI group, TBI+Negative control (NC) group, TBI+Sensory integration (SI) group, TBI+Atypical chemokine receptor 2 (ACKR2) group and TBI+SI+ACKR2 group. We employed several techniques, including the water maze test, transmission electron microscope, HE staining, enzyme-linked immunosorbent assay, RT-PCR, western blotting to elucidate the role of ACKR2 in young rats with traumatic brain injury.
Results: We observed that the ability of learning and memory were significantly decreased, the serum inflammatory factors CCL2, IL-1β and TNF-α were increased, the expression of Tau and NG2 was increased, and the expression of ACKR2 was decreased in TBI rats. After ACKR2 overexpression combined with SI, the learning ability and memory ability of TBI rats were improved, the levels of CCL2, IL-1β, TNF-α were decreased, the expressions of Tau and NG2 were decreased, which were better than those of SI and ACKR2 groups.
Conclusion: ACKR2 may be an effective method for improving the results after TBI in young rats.
Keywords: ACKR2; cognitive impairment; traumatic brain injury.
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