A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation

Andrey Formozov; Alexander Dieter; J Simon Wiegert

doi:10.1016/j.crmeth.2023.100418

A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation

Cell Rep Methods. 2023 Mar 7;3(3):100418. doi: 10.1016/j.crmeth.2023.100418. eCollection 2023 Mar 27.

Authors

Andrey Formozov^{1

2}, Alexander Dieter^{1

2}, J Simon Wiegert^{1

2}

Affiliations

¹ Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
² Department of Neurophysiology, MCTN, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.

Abstract

Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools in vivo at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.

Keywords: all-optical; fiber photometry; fused fiber coupler; genetically encoded indicators; hippocampus; in vivo; mouse brain; neuroscience; open source; optogenetics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain* / physiology
Optogenetics*
Photometry

Associated data

figshare/10.6084/m9.figshare.21960050