Rapid sterilisation and diabetic cutaneous regeneration using cascade bio-heterojunctions through glucose oxidase-primed therapy

Yi Deng; Xumei Ouyang; Jiyu Sun; Xiuyuan Shi; Yunfei Li; Yau Kei Chan; Weizhong Yang; Shaojun Peng

doi:10.1016/j.bioactmat.2022.07.003

Rapid sterilisation and diabetic cutaneous regeneration using cascade bio-heterojunctions through glucose oxidase-primed therapy

Bioact Mater. 2022 Jul 31:25:748-765. doi: 10.1016/j.bioactmat.2022.07.003. eCollection 2023 Jul.

Authors

Yi Deng^{1

2

3}, Xumei Ouyang⁴, Jiyu Sun⁵, Xiuyuan Shi⁶, Yunfei Li⁷, Yau Kei Chan⁸, Weizhong Yang¹, Shaojun Peng⁴

Affiliations

¹ College of Biomedical Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
² State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China.
³ Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, China.
⁴ Zhuhai Institute of Translational Medicine, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, Guangdong, 519000, China.
⁵ State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
⁶ Department of Materials, Imperial College London, SW7 2AZ, London, United Kingdom.
⁷ Department of Biomedical Engineering, The City College of the City University of New York, New York, United States.
⁸ Department of Ophthalmology, The University of Hong Kong, Hong Kong, China.

Abstract

The cutaneous wound in diabetic patients frequently encounters intractable pathogenic infections due to the hyperglycemia micromilieu which is conducive to bacterial growth and multiplication. Despite the extensive clinical use of antibiotics to treat bacterial infections, the emergence of drug-resistant and super pathogens as well as the potential side effects of antibiotics have elicited alarming challenges to public health. To address this daunting concern, we devise and develop a photo-activated cascade bio-heterojunctions (C-bio-HJs) for rapid sterilization and diabetic cutaneous regeneration. In the designed C-bio-HJs, photo-generated electron-hole pairs of graphite-phase carbon nitride (g-C₃N₄) are effectively separated with the marriage of molybdenum disulfide (MoS₂), which achieves the augmented photodynamic antibacterial effect. Moreover, glucose oxidase (GOx) tethered on the bio-HJs catalyzes glucose into hydrogen peroxide (H₂O₂) in diabetic wounds for starvation therapy. Furthermore, Mo⁴⁺ enables the catalysis of H₂O₂ into a highly effective hydroxyl radical (·OH) for chemodynamic-photothermal combined antibacterial therapy. Both in vitro and in vivo results authenticate the cascading antibacterial properties and skin regeneration-promoting effects of the C-bio-HJs, which provide a facile strategy to combat diabetic wound healing through the synergistic GOx-primed dynamic therapies.

Keywords: Antibacterial therapy; Bio-heterojunction; Rapid sterilization; Tissue regeneration; g-C3N4.