Bone/cartilage organoid on-chip: Construction strategy and application

Yan Hu; Hao Zhang; Sicheng Wang; Liehu Cao; Fengjin Zhou; Yingying Jing; Jiacan Su

doi:10.1016/j.bioactmat.2023.01.016

Bone/cartilage organoid on-chip: Construction strategy and application

Bioact Mater. 2023 Jan 20:25:29-41. doi: 10.1016/j.bioactmat.2023.01.016. eCollection 2023 Jul.

Authors

Yan Hu^{1

2

3

4}, Hao Zhang^{1

2

3}, Sicheng Wang⁵, Liehu Cao⁶, Fengjin Zhou⁷, Yingying Jing^{1

3}, Jiacan Su^{1

2

3}

Affiliations

¹ Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
² Department of Orthopaedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
³ Musculoskeletal Organoid Research Center, Shanghai University, Shanghai, 200444, China.
⁴ Shaoxing Institute of Technology at Shanghai University, Shaoxing, 312000, China.
⁵ Department of Orthopaedics, Shanghai Zhongye Hospital, Shanghai, 201941, China.
⁶ Department of Orthopaedics, Shanghai Baoshan Luodian Hospital, Shanghai, 201908, China.
⁷ Department of Orthopaedics, Honghui Hospital, Xi'an Jiao Tong University, Xi'an, 710000, China.

Abstract

The necessity of disease models for bone/cartilage related disorders is well-recognized, but the barrier between ex-vivo cell culture, animal models and the real human body has been pending for decades. The organoid-on-a-chip technique showed opportunity to revolutionize basic research and drug screening for diseases like osteoporosis and arthritis. The bone/cartilage organoid on-chip (BCoC) system is a novel platform of multi-tissue which faithfully emulate the essential elements, biologic functions and pathophysiological response under real circumstances. In this review, we propose the concept of BCoC platform, summarize the basic modules and current efforts to orchestrate them on a single microfluidic system. Current disease models, unsolved problems and future challenging are also discussed, the aim should be a deeper understanding of diseases, and ultimate realization of generic ex-vivo tools for further therapeutic strategies of pathological conditions.

Keywords: Ex-vivo disease model; On-chip platform; Organoid; Osteochondral unit.

Publication types

Review