A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines

Giovani Trevisan; Drew Magstadt; Amy Woods; Joel Sparks; Michael Zeller; Ganwu Li; Karen M Krueger; Anugrah Saxena; Jianqiang Zhang; Phillip C Gauger

doi:10.3389/fvets.2023.1149293

A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines

Front Vet Sci. 2023 Mar 28:10:1149293. doi: 10.3389/fvets.2023.1149293. eCollection 2023.

Authors

Affiliations

¹ Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, United States.
² AMVC, Frankfort, IN, United States.
³ Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.

Abstract

A porcine reproductive and respiratory syndrome virus (PRRSV) type 2 (PRRSV-2) isolate was obtained from lung samples collected from a 4.5-month-old pig at a wean-to-finish site in Indiana, USA, although no gross or microscopic lesions suggestive of PRRSV infection were observed in the lung tissue. Phylogenetic and molecular evolutionary analyses based on the obtained virus sequences indicated that PRRSV USA/IN105404/2021 was a natural recombinant isolate from Ingelvac PRRS^® MLV and Prevacent^® PRRS, which are PRRSV-2-modified live virus vaccines commercially available in the United States. This study is the first to report the detection of a PRRSV-2 recombinant strain consisting entirely of two modified live virus vaccine strains under field conditions. Based on clinical data and the absence of lung lesions, this PRRSV-2 recombinant strain was not virulent in swine, although its pathogenicity needs to be confirmed by clinical trials.

Keywords: MLV vaccines; NGS; PRRSV; evolution; recombination.

Grants and funding

Funding for the subsequent virus investigation of the clinical case and the analysis performed under this project was provided by the Iowa State University Veterinary Diagnostic Laboratory.