Down-regulation of human long non-coding RNA LINC01187 is associated with nephropathies

Theodora Manolakou; Valeria Kaltezioti; Niki Prakoura; Panagiotis Kavvadas; Simone Reichelt-Wurm; Harikleia Gakiopoulou; Miriam Banas; Bernhard Banas; Maja T Lindenmeyer; Clemens D Cohen; Peter Boor; Sonja Djudjaj; Dimitrios T Boumpas; Christos Chatziantoniou; Aristidis Charonis; Panagiotis K Politis

doi:10.1111/jcmm.17014

Down-regulation of human long non-coding RNA LINC01187 is associated with nephropathies

J Cell Mol Med. 2023 May;27(9):1192-1205. doi: 10.1111/jcmm.17014. Epub 2023 Apr 13.

Authors

Theodora Manolakou^{1

2

3}, Valeria Kaltezioti^{1

2}, Niki Prakoura⁴, Panagiotis Kavvadas⁴, Simone Reichelt-Wurm⁵, Harikleia Gakiopoulou⁶, Miriam Banas⁵, Bernhard Banas⁵, Maja T Lindenmeyer⁷, Clemens D Cohen⁸, Peter Boor^{9

10}, Sonja Djudjaj⁹, Dimitrios T Boumpas^{2

11}, Christos Chatziantoniou^{4

12

13}, Aristidis Charonis^{2

14}, Panagiotis K Politis¹

Affiliations

¹ Center for Basic Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
² Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
³ School of Medicine, National and Kapodistrian University of Athens, Greece.
⁴ Batiment Recherche, Tenon Hospital, Paris, France.
⁵ Department of Nephrology, University Hospital Regensburg, Regensburg, Germany.
⁶ 1st Department of Pathology, School of Medicine, National & Kapodistrian University of Athens, Athens, Greece.
⁷ III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
⁸ Nephrological Center, Medical Clinic and Policlinic IV, University Hospital of Munich, Munich, Germany.
⁹ Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany.
¹⁰ Department of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany.
¹¹ Rheumatology and Clinical Immunology Unit, 4th Department of Internal Medicine, Attikon University Hospital, Joint Rheumatology Program, National and Kapodistrian University of Athens Medical School, Athens, Greece.
¹² Faculty of Medicine, Sorbonne University, Paris, France.
¹³ Institut National de la Santé et de la Recherche Médicale (INSERM) Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France.
¹⁴ University Research Institute of Maternal and Child Health and Precision Medicine, Athens, Greece.

Abstract

Chronic kidney diseases affect a substantial percentage of the adult population worldwide. This observation emphasizes the need for novel insights into the molecular mechanisms that control the onset and progression of renal diseases. Recent advances in genomics have uncovered a previously unanticipated link between the non-coding genome and human kidney diseases. Here we screened and analysed long non-coding RNAs (lncRNAs) previously identified in mouse kidneys by genome-wide transcriptomic analysis, for conservation in humans and differential expression in renal tissue from healthy and diseased individuals. Our data suggest that LINC01187 is strongly down-regulated in human kidney tissues of patients with diabetic nephropathy and rapidly progressive glomerulonephritis, as well as in murine models of kidney diseases, including unilateral ureteral obstruction, nephrotoxic serum-induced glomerulonephritis and ischemia/reperfusion. Interestingly, LINC01187 overexpression in human kidney cells in vitro inhibits cell death indicating an anti-apoptotic function. Collectively, these data suggest a negative association of LINC01187 expression with renal diseases implying a potential protective role.

Keywords: Gm12121; LINC01187; apoptosis; in situ hybridization; long non-coding RNAs; renal diseases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diabetic Nephropathies* / metabolism
Down-Regulation / genetics
Glomerulonephritis* / metabolism
Humans
Kidney / metabolism
Mice
RNA, Long Noncoding* / metabolism

Substances

RNA, Long Noncoding