Chitosan and chitooligosaccharide regulated reactive oxygen species homeostasis at wounds of pear fruit during healing

Abstract

Both chitosan (CTS) and chitooligosaccharide (COS) can promote fruit healing. However, whether the two chemicals regulate reactive oxygen species (ROS) homeostasis during wound healing of pear fruit remains unknown. In this study, the wounded pear fruit (Pyrus bretschneideri cv. Dongguo) was treated with a 1 g L^-1 CTS and COS. We found CTS and COS treatments increased NADPH oxidase and superoxide dismutase activities, and promoted O₂^.- and H₂O₂ production at wounds. CTS and COS also enhanced the activities of catalase, peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, and elevated the levels of ascorbic acid and glutathione. In addition, the two chemicals improved antioxidant capacity in vitro and maintained cell membrane integrity at fruit wounds during healing. Taken together, CTS and COS can regulate ROS homeostasis at wounds of pear fruit during healing by scavenging excessive H₂O₂ and improving antioxidant capacity. Overall, the COS demonstrated superior performance over the CTS.

Keywords: Antioxidant capacity; Chitooligosaccharide; Chitosan; Pyrus bretschneideri; Reactive oxygen species homeostasis; Wound healing.