Nitrogen and sulfur co-doped photoluminescent carbon dots for highly selective and sensitive detection of Ag+ and Hg2+ ions in aqueous media: Applications in bioimaging and real sample analysis

Abstract

In this study, we report the synthesis of photoluminescent (PL) nitrogen (N) and sulfur (S) co-doped carbon dots (NS-CDs) from nitazoxanide and 3-mercaptopropionic acid as a precursors via a one-pot hydrothermal methods. N and S co-doped materials allows more active sites in the CDs surface resulting in an enhancement of their PL properties. NS-CDs show bright blue PL, excellent optical properties, good water solubility, and a high quantum yield (QY) of 32.1%. The as-prepared NS-CDs were confirmed by UV-Visible, photoluminescence, FTIR, XRD and TEM analysis. An optimized excitation at 345 nm, the NS-CDs exhibited strong PL emission at 423 nm with an average size of 3.53 ± 0.25 nm. Under optimized conditions, the NS-CDs PL probe shows high selectivity with Ag⁺/Hg²⁺ ions detected, while other cations no significant changes the PL signal. The PL intensity of NS-CDs linearly quenching and enhancement with Ag⁺ and Hg²⁺ ions from 0 to 50 × 10^-6 M, with the detection limit of 2.15 × 10^-6 M and 6.77 × 10^-7 M (S/N = 3). More interestingly, as-synthesized NS-CDs shows a strong binding to Ag⁺/Hg²⁺ ions with the PL quenching and enhancement to precise and quantitative detection of Ag⁺/Hg²⁺ ions in living cells. The proposed system was effectively utilized for the sensing of Ag⁺/Hg²⁺ ions in real samples resulting in high sensitivity and good recoveries (98.4-109.7%).

Keywords: Ag(+) and Hg(2+) ions; Bioimaging; Carbon dots; Chemosensors; Ratiometric; Real sample.