Proteomics mining of cancer hallmarks on a single-cell resolution

Maomao Li; Jing Zuo; Kailin Yang; Ping Wang; Shengtao Zhou

doi:10.1002/mas.21842

Proteomics mining of cancer hallmarks on a single-cell resolution

Mass Spectrom Rev. 2023 Apr 12. doi: 10.1002/mas.21842. Online ahead of print.

Authors

Maomao Li¹, Jing Zuo², Kailin Yang³, Ping Wang¹, Shengtao Zhou¹

Affiliations

¹ Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE and State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, Sichuan, China.
² State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, and West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan, China.
³ Department of Radiation Oncology, Taussig Cancer Center, Cleveland Clinic, Cleveland, Ohio, USA.

PMID: 37051664
DOI: 10.1002/mas.21842

Abstract

Dysregulated proteome is an essential contributor in carcinogenesis. Protein fluctuations fuel the progression of malignant transformation, such as uncontrolled proliferation, metastasis, and chemo/radiotherapy resistance, which severely impair therapeutic effectiveness and cause disease recurrence and eventually mortality among cancer patients. Cellular heterogeneity is widely observed in cancer and numerous cell subtypes have been characterized that greatly influence cancer progression. Population-averaged research may not fully reveal the heterogeneity, leading to inaccurate conclusions. Thus, deep mining of the multiplex proteome at the single-cell resolution will provide new insights into cancer biology, to develop prognostic biomarkers and treatments. Considering the recent advances in single-cell proteomics, herein we review several novel technologies with particular focus on single-cell mass spectrometry analysis, and summarize their advantages and practical applications in the diagnosis and treatment for cancer. Technological development in single-cell proteomics will bring a paradigm shift in cancer detection, intervention, and therapy.

Keywords: cancer hallmarks; intratumoral heterogeneity; mass spectrometry; single-cell proteomics.

Publication types

Review