Spearmint essential oil (SEO), one of the economically valuable natural products, has special importance in the food, pharmaceutical, and perfumery industries due to its antifungal, antioxidant, and enzyme inhibitory properties. In this study, we optimized and evaluated the effect of three pre-treatments on the extraction of SEO for quantity and quality: ultrasonic bath (UB), water to material ratio-ultrasonic bath (W/M-UB), and cold plasma-ultrasonic bath (CP-UB). Three experiments were designed using the central composite design (CCD) of response surface methodology (RSM). Experimental treatments included UB temperature (30-80°C) and time duration (1-30 min), cold plasma (CP) power (15-24 kV), and water/material ratio (10-40). Then, SEOs were extracted by hydro-distillation using the Clevenger apparatus. The results showed that SEO yield in the optimal conditions of treatments was 119.7%, 206.6%, and 155.7% higher in UB, W/M-UB, and CP-UB pretreatments respectively, in comparison to control sample and optimized conditions were UB temperature: 37.3°C and UB time: 5.2 min at UB treatment, 33.9 of W/M ratio, 69.9°C of UB temperature and 6.9 min of UB time at W/M-UB treatment and CP power: 22.176, UB temperature: 40.135 and UB time: 24.122 at CP-UB treatment. Oxygenated monoterpenes were also higher in the essential oils (EOs) of all three treated plant materials. In conclusion, the SEO extraction yield improved by the application of the pretreatments in optimized conditions.
Keywords: cold plasma; hydro‐distillation; optimization; spearmint; ultrasonic.
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