Towards Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole for the Detection of Bacteria- Listeria monocytogenes

Viktorija Liustrovaite; Maksym Pogorielov; Raimonda Boguzaite; Vilma Ratautaite; Almira Ramanaviciene; Greta Pilvenyte; Viktoriia Holubnycha; Viktoriia Korniienko; Kateryna Diedkova; Roman Viter; Arunas Ramanavicius

doi:10.3390/polym15071597

Towards Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole for the Detection of Bacteria- Listeria monocytogenes

Polymers (Basel). 2023 Mar 23;15(7):1597. doi: 10.3390/polym15071597.

Authors

Viktorija Liustrovaite¹, Maksym Pogorielov^{2

3}, Raimonda Boguzaite^{4

5}, Vilma Ratautaite^{4

5}, Almira Ramanaviciene⁵, Greta Pilvenyte⁴, Viktoriia Holubnycha², Viktoriia Korniienko^{2

3}, Kateryna Diedkova^{2

3}, Roman Viter³, Arunas Ramanavicius^{1

4}

Affiliations

¹ Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.
² Biomedical Research Centre, Sumy State University, R-Korsakova Street, 40007 Sumy, Ukraine.
³ Institute of Atomic Physics and Spectroscopy, University of Latvia, Jelgavas iela 3, LV-1004 Riga, Latvia.
⁴ Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, Saulėtekio Av. 3, LT-10257 Vilnius, Lithuania.
⁵ NanoTechnas-Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.

Abstract

Detecting bacteria-Listeria monocytogenes-is an essential healthcare and food industry issue. The objective of the current study was to apply platinum (Pt) and screen-printed carbon (SPCE) electrodes modified by molecularly imprinted polymer (MIP) in the design of an electrochemical sensor for the detection of Listeria monocytogenes. A sequence of potential pulses was used to perform the electrochemical deposition of the non-imprinted polypyrrole (NIP-Ppy) layer and Listeria monocytogenes-imprinted polypyrrole (MIP-Ppy) layer over SPCE and Pt electrodes. The bacteria were removed by incubating Ppy-modified electrodes in different extraction solutions (sulphuric acid, acetic acid, L-lysine, and trypsin) to determine the most efficient solution for extraction and to obtain a more sensitive and repeatable design of the sensor. The performance of MIP-Ppy- and NIP-Ppy-modified electrodes was evaluated by pulsed amperometric detection (PAD). According to the results of this research, it can be assumed that the most effective MIP-Ppy/SPCE sensor can be designed by removing bacteria with the proteolytic enzyme trypsin. The LOD and LOQ of the MIP-Ppy/SPCE were 70 CFU/mL and 210 CFU/mL, respectively, with a linear range from 300 to 6700 CFU/mL.

Keywords: L-lysine; Listeria monocytogenes; acetic acid; molecularly imprinted polymer; molecularly imprinted polypyrrole; pulsed amperometric detection; sulphuric acid; template extraction method; trypsin; whole-cell imprinting.

Grants and funding

Research was funded under the CFLA project “Jauna fotoluminescences platforma Listeria monocitogēnu noteikšanai” (1.1.1.5/21/A/001). L. monocytogenes samples were prepared under the support from LV-UA bilateral project (Ministry of Educaton and Science of Ukraine) “Development of nanostructured optical sensor system for detection of K. pneumonia” (LV-UA/2021/2).