Role of Na+,K+-ATPase in Bone Remodeling

N A Pasatetskaya; S I Klimshin; E V Lopatina

doi:10.1007/s10517-023-05768-4

Role of Na⁺,K⁺-ATPase in Bone Remodeling

Bull Exp Biol Med. 2023 Mar;174(5):678-680. doi: 10.1007/s10517-023-05768-4. Epub 2023 Apr 13.

Authors

N A Pasatetskaya¹, S I Klimshin², E V Lopatina^{2

3}

Affiliations

¹ I. P. Pavlov First St. Petersburg State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia. npasatetckaia@yandex.ru.
² I. P. Pavlov First St. Petersburg State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
³ I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.

PMID: 37046115
DOI: 10.1007/s10517-023-05768-4

Abstract

The effects of cardiotonic steroids (ouabain and digoxin) on the bone formation were studied using the organotypic tissue culture in combination with confocal microscopy. The expression of α₁- and α₃-isoforms of Na⁺,K⁺-ATPase was detected in cells of the bone tissue of 12-day-old chicken embryos. Ouabain in a concentrations 10^-10 M (comparable with its endogenous concentration) can modulate transducer function of Na⁺,K⁺-ATPase and control the growth and proliferation bone tissue cells. Unlike ouabain, digoxin is not involved in the regulation of bone tissue growth in a 12-day-old chicken embryo.

Keywords: Na+,K+-ATPase; bone tissue; digoxin; organotypic culture; ouabain.

MeSH terms

Animals
Bone Remodeling
Chick Embryo
Digoxin / pharmacology
Ouabain* / pharmacology
Protein Isoforms / metabolism
Sodium
Sodium-Potassium-Exchanging ATPase* / metabolism

Substances

Ouabain
Sodium-Potassium-Exchanging ATPase
Digoxin
Protein Isoforms
Sodium