Two new populations of Pyrgulopsis were found in springs in the Chihuahuan desert region of Texas. Pyrgulopsis Call & Pilsbry, 1886 are a diverse group of snails occupying freshwater springs, usually narrow-range endemics limited to a single spring. Their small ranges confer substantial conservation concern as the drying or disturbance of a single spring can lead to their extinction. We use mitochondrial COI and nuclear LSU sequences, shell morphometrics, and anatomical features to distinguish a new species of Pyrgulopsis endemic to the Big Bend region of Texas.