Ecdysone signaling determines lateral polarity and remodels neurites to form Drosophila's left-right brain asymmetry

So Sakamura; Fu-Yu Hsu; Akari Tsujita; Mohammed Bin Abubaker; Ann-Shyn Chiang; Kenji Matsuno

doi:10.1016/j.celrep.2023.112337

Ecdysone signaling determines lateral polarity and remodels neurites to form Drosophila's left-right brain asymmetry

Cell Rep. 2023 Apr 25;42(4):112337. doi: 10.1016/j.celrep.2023.112337. Epub 2023 Apr 11.

Authors

So Sakamura¹, Fu-Yu Hsu², Akari Tsujita¹, Mohammed Bin Abubaker³, Ann-Shyn Chiang⁴, Kenji Matsuno⁵

Affiliations

¹ Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
² Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan; Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan.
³ Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan.
⁴ Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan; Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan; Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80780, Taiwan; Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli 35053, Taiwan; Graduate Institute of Clinical Medical Science, China Medical University, Taichung 40402, Taiwan; Kavli Institute for Brain and Mind, University of California San Diego, La Jolla, CA 92093-0526, USA.
⁵ Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan. Electronic address: kmatsuno@bio.sci.osaka-u.ac.jp.

PMID: 37044096
DOI: 10.1016/j.celrep.2023.112337

Abstract

Left-right (LR) asymmetry of the brain is fundamental to its higher-order functions. The Drosophila brain's asymmetrical body (AB) consists of a structural pair arborized from AB neurons and is larger on the right side than the left. We find that the AB initially forms LR symmetrically and then develops LR asymmetrically by neurite remodeling that is specific to the left AB and is dynamin dependent. Additionally, neuronal ecdysone signaling inhibition randomizes AB laterality, suggesting that ecdysone signaling determines AB's LR polarity. Given that AB's LR asymmetry relates to memory formation, our research establishes AB as a valuable model for studying LR asymmetry and higher-order brain function relationships.

Keywords: CP: Neuroscience; Drosophila; asymmetrical body; brain; ecdysone signaling; left-right asymmetry; neurite remodeling; pruning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Body Patterning / physiology
Brain
Drosophila
Ecdysone*
Neurites*
Signal Transduction

Substances

Ecdysone