Electrochemical immunosensing by carbon ink/carbon dot/ZnO-labeled-Ag@polypyrrole composite biomarker for CA-125 ovarian cancer detection

Nattinee Krathumkhet; Toyoko Imae; Fu-Ming Wang; Chiou-Chung Yuan; Jusni Manidae Lumban Gaol; Nophawan Paradee

doi:10.1016/j.bioelechem.2023.108430

Electrochemical immunosensing by carbon ink/carbon dot/ZnO-labeled-Ag@polypyrrole composite biomarker for CA-125 ovarian cancer detection

Bioelectrochemistry. 2023 Aug:152:108430. doi: 10.1016/j.bioelechem.2023.108430. Epub 2023 Mar 31.

Authors

Nattinee Krathumkhet¹, Toyoko Imae², Fu-Ming Wang³, Chiou-Chung Yuan⁴, Jusni Manidae Lumban Gaol⁵, Nophawan Paradee⁶

Affiliations

¹ Sustainable Polymer & Innovative Composite Materials Research Group, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Road, Bangmod, Thungkru, Bangkok 10140, Thailand.
² Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC; Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC; Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC. Electronic address: imae@mail.ntust.edu.tw.
³ Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC; Sustainable Energy Center, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC; Department of Chemical Engineering, Chung Yuan Christian University, 200 Zhongbei Rd, Zhongli District, Taoyuan 32023, Taiwan, ROC; R&D Center for Membrane Technology, Chung Yuan Christian University, 200 Zhongbei Rd, Zhongli District, Taoyuan 32023, Taiwan, ROC. Electronic address: mccabe@mail.ntust.edu.tw.
⁴ Department of Obstetrics and Gynecology, Cheng Hsin General Hospital, 45 Zhenxing St, Beitou, Taipei 11220, Taiwan, ROC.
⁵ Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC.
⁶ Sustainable Polymer & Innovative Composite Materials Research Group, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Road, Bangmod, Thungkru, Bangkok 10140, Thailand. Electronic address: nophawan.par@kmutt.ac.th.

PMID: 37043880
DOI: 10.1016/j.bioelechem.2023.108430

Abstract

In this work, we demonstrated a novel cancer antigen 125 (CA125) biomarker detection based on electrochemical immunosensor. The biomarker on conductive composite materials of carbon ink/carbon dot/zine oxide (C-ink/CD/ZnO) was employed as an electrode platform by using ITO substrate to enhance the interaction of antibodies (Ab) with supporting catalytic performance of ZnO as a labeling signal molecule. They were a scientist attention for biosensor with chemical stability, strong biocompatibility, high conductive signal, and accuracy. Moreover, the nanocomposite of silver@polypyrrole (Ag@PPy) was used as a potential redox mediator. The labeled construction with Ag@PPy was more accuracy than that of a free-labeled. The created immunosensor was a wide linear range as 1 ag·mL^-1 - 100 ng·mL^-1 and a low limitation of detection as 0.1 fg·mL^-1 under the optimal condition. This suggested that the immunosensor is considered to be an accurate and efficient diagnostic tool for CA125 and other biomarkers detection in actual sample analysis for clinic.

Keywords: Cancer antigen 125; Electrochemical immunosensor; Ovarian cancer; Silver@polypyrrle nanoparticles; Zinc oxide.

MeSH terms

Biosensing Techniques*
CA-125 Antigen / analysis
Carbon / chemistry
Electrochemical Techniques
Female
Humans
Immunoassay
Ink
Limit of Detection
Metal Nanoparticles* / chemistry
Ovarian Neoplasms*
Polymers / chemistry
Pyrroles
Zinc Oxide*

Substances

Carbon
polypyrrole
Polymers
Zinc Oxide
Pyrroles
CA-125 Antigen