Puncture alignments are found on Palaeolithic carvings, pendants, and other fully shaped osseous artifacts. These marks were interpreted as abstract decorations, system of notations, and features present on human and animal depictions. Here, we create an experimental framework for the analysis and interpretation of human-made punctures and apply it to a highly intriguing, punctured bone fragment found at Canyars, an Early Upper Palaeolithic coastal site from Catalonia, Spain. Changes of tool and variation in the arrangement and orientation of punctures are consistent with the interpretation of this object as the earliest-known leather work punch board recording six episodes of hide pricking, one of which was to produce a linear seam. Our results indicate that Aurignacian hunters-gatherers used this technology to produce leather works and probably tailored clothes well before the introduction of bone eyed needles in Europe 15,000 years later.