In this Perspective, we present examples of isotope effects that originate from noncovalent interactions, mainly hydrogen bonding, electrostatics, and confinement. They are traditionally widely used in isotopic enrichment processes, as well as in studies of mechanisms of different (bio)chemical and physical phenomena. We then show the emerging areas of their applications, mainly medical and material sciences. We stress that these emerging applications require either high enrichment or isotopic substitution, which requires the development of new effective techniques of isotopic purification.