Pushing the Boundaries of Structural Heterogeneity with the Lipid A of Marine Bacteria Cellulophaga

Roberta Cirella; Chiara Pagliuca; Molly Dorothy Pither; Elena Scaglione; Olga I Nedashkovskaya; Oleg V Chernikov; Kuo-Feng Hua; Roberta Colicchio; Mariateresa Vitiello; Maxim S Kokoulin; Alba Silipo; Paola Salvatore; Antonio Molinaro; Flaviana Di Lorenzo

doi:10.1002/cbic.202300183

Pushing the Boundaries of Structural Heterogeneity with the Lipid A of Marine Bacteria Cellulophaga

Chembiochem. 2023 May 16;24(10):e202300183. doi: 10.1002/cbic.202300183. Epub 2023 Apr 21.

Authors

Roberta Cirella¹, Chiara Pagliuca², Molly Dorothy Pither¹, Elena Scaglione², Olga I Nedashkovskaya³, Oleg V Chernikov³, Kuo-Feng Hua⁴, Roberta Colicchio², Mariateresa Vitiello², Maxim S Kokoulin³, Alba Silipo^{1

5}, Paola Salvatore^{2

5

6}, Antonio Molinaro^{1

5}, Flaviana Di Lorenzo^{1

5}

Affiliations

¹ Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy.
² Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini, 5, 80131, Naples, Italy.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, 690022, Vladivostok, Russia.
⁴ Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shen-Lung Road, Ilan, 26099, Taiwan.
⁵ Task Force on Microbiome Studies, University of Naples Federico II.
⁶ CEINGE-Biotecnologie Avanzate Franco Salvatore s.c.ar.l., Via G. Salvatore, 436, 80131, Naples, Italy.

PMID: 37042436
DOI: 10.1002/cbic.202300183

Abstract

Marine bacteria, which are often described as chemical gold, are considered an exceptional source of new therapeutics. Considerable research interest has been given to lipopolysaccharides (LPSs), the main components of the Gram-negative outer membrane. LPS and its lipid A portion from marine bacteria are known to exhibit a tricky chemistry that has been often associated with intriguing properties such as behaving as immune adjuvants or anti-sepsis molecules. In this scenario, we report the structural determination of the lipid A from three marine bacteria within the Cellulophaga genus, which showed to produce an extremely heterogenous blend of tetra- to hexa-acylated lipid A species, mostly carrying one phosphate and one D-mannose on the glucosamine disaccharide backbone. The ability of the three LPSs in activating TLR4 signaling revealed a weaker immunopotential by C. baltica NNO 15840^T and C. tyrosinoxydans EM41^T , while C. algicola ACAM 630^T behaved as a more potent TLR4 activator.

Keywords: Cellulophaga; Lipid A; Lipopolysaccharide (LPS); MALDI-TOF mass spectrometry; Marine bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flavobacteriaceae*
Gammaproteobacteria*
Lipid A / chemistry
Lipopolysaccharides / chemistry
Toll-Like Receptor 4

Substances

Lipid A
Toll-Like Receptor 4
Lipopolysaccharides