A Brief Practical Guide to PCR

Page B McKinzie; Meagan B Myers

doi:10.1007/978-1-0716-2950-5_1

A Brief Practical Guide to PCR

Methods Mol Biol. 2023:2621:3-13. doi: 10.1007/978-1-0716-2950-5_1.

Authors

Page B McKinzie¹, Meagan B Myers²

Affiliations

¹ Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA. page.mckinzie@fda.hhs.gov.
² Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA. meagan.myers@fda.hhs.gov.

PMID: 37041436
DOI: 10.1007/978-1-0716-2950-5_1

Abstract

Polymerase chain reaction (PCR) has been a powerful molecular biology tool since the mid-1980s. Millions of copies of specific sequence regions of DNA can be generated to allow the study of these regions. Fields that use this technology range from forensics to the experimental study of human biology. Standards for performing PCR and information tools to help design PCR protocols aid in successful implementation of PCR.

Keywords: Oligonucleotide; Polymerase chain reaction; Q5; Taq.

MeSH terms

DNA*
Humans
Polymerase Chain Reaction / methods
Taq Polymerase

Substances

Taq Polymerase
DNA