Somatic Cell Nuclear Transfer in Rabbits

Pengxiang Qu; Wenbin Cao; Enqi Liu

doi:10.1007/978-1-0716-3064-8_8

Somatic Cell Nuclear Transfer in Rabbits

Methods Mol Biol. 2023:2647:169-181. doi: 10.1007/978-1-0716-3064-8_8.

Authors

Pengxiang Qu^{1

2}, Wenbin Cao^{1

2}, Enqi Liu^{3

4}

Affiliations

¹ Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
² Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an, Shaanxi, China.
³ Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China. liuenqi@xjtu.edu.cn.
⁴ Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi'an, Shaanxi, China. liuenqi@xjtu.edu.cn.

PMID: 37041334
DOI: 10.1007/978-1-0716-3064-8_8

Abstract

Somatic cell nuclear transfer (SCNT) is a technology that enables differentiated somatic cells to acquire a totipotent state, thus making it of great value in developmental biology, biomedical research, and agricultural applications. Rabbit cloning associated with transgenesis has the potential to improve the applicability of this species for disease modeling, drug testing, and production of human recombinant proteins. In this chapter, we introduce our SCNT protocol for the production of live cloned rabbits.

Keywords: Cloning; Embryo; Nuclear transplantation; Oryctolagus cuniculus; Rabbit; Somatic cell nuclear transfer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Cloning, Organism* / methods
Gene Transfer Techniques
Humans
Nuclear Transfer Techniques*
Rabbits