Vibrio paucivorans sp. nov. and Vibrio qingdaonensis sp. nov. , two marine bacteria

Abstract

Two Gram-stain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacteria, designated strains DBSS07^T and ZSDZ65^T, were isolated from the surface sediments of the Bohai sea and Qingdao coastal seawater, respectively. Phylogenetic analysis based on 16S rRNA genes, multilocus sequence analysis (MLSA), phylogenomic analysis of single-copy gene families and whole-genome data placed DBSS07^T and ZSDZ65^T within the genus Vibrio. DBSS07^T was most closely related to Vibrio aestivus M22^T, with 97.51 % sequence similarity, whereas ZSDZ65^T was most closely related to Vibrio variabilis R-40492^T with 97.58 % sequence similarity. DBSS07^T grew with 1-7 % (w/v) NaCl (optimum 3 %), at 16-37 °C (optimum 28 °C) and at pH 6.0-9.0 (optimum pH 7.0); whereas ZSDZ65^T grew with 1-5 % (w/v) NaCl (optimum 2 %), at 16-32 °C (optimum 28 °C) and at pH 6.0-9.0 (optimum pH 8.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (C_{16 : 1}ω7c or/and C_{16 : 1}ω6c), with different proportions. The DNA G+C contents of DBSS07^T and ZSDZ65^T were 44.7 and 44.3 %, respectively. On the basis of the results of polyphasic analysis, DBSS07^T and ZSDZ65^T are considered to represent novel species within the genus Vibrio, for which the names V. paucivorans sp. nov. (type strain, DBSS07^T = KCTC 82896^T= MCCC 1K06284^T) and V. qingdaonensis sp. nov. (type strain, ZSDZ65^T = KCTC 82893^T = MCCC 1K06289^T) are proposed, respectively.

Keywords: Bohai sea; Qingdao coastal seawater; Vibrio paucivorans sp. nov; Vibrio qingdaonensis sp. nov; surface sediment.