Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading

Kiyoon Min; Giyoong Tae

doi:10.1186/s40824-023-00373-y

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading

Biomater Res. 2023 Apr 10;27(1):28. doi: 10.1186/s40824-023-00373-y.

Authors

Kiyoon Min¹, Giyoong Tae²

Affiliations

¹ School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-Gwagiro, Buk-Gu, Gwangju, 61005, Republic of Korea.
² School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-Gwagiro, Buk-Gu, Gwangju, 61005, Republic of Korea. gytae@gist.ac.kr.

Abstract

Background: Cellular infiltration and angiogenesis into implanted biomaterial scaffolds are crucial for successful host tissue integration and tissue regeneration. Cellulose nanocrystal (CNC) is a nano-sized cellulose derivative, which can form an injectable physical gel with salts. Sulfate groups of sulfated CNC (CNC-S) can act as a binding domain to various growth factors and cytokines with a heparin-binding domain for sustained release of them. Vascular endothelial growth factor (VEGF) can promote the proliferation of endothelial cells and angiogenesis. In this study, VEGF-loaded CNC-S hydrogel was evaluated as an injectable scaffold that can induce cellular infiltration and angiogenesis.

Methods: CNC-S was hydrolyzed to get desulfated CNC (CNC-DS), which was used as a negative control group against CNC-S. Both CNC-S and CNC-DS hydrogels were prepared and compared in terms of biocompatibility and VEGF release. The hydrogels with or without VEGF loading were subcutaneously injected into mice to evaluate the biocompatibility, cellular infiltration, and angiogenesis induction of the hydrogels.

Results: Both hydrogels possessed similar stability and shear-thinning behavior to be applicable as injectable hydrogels. However, CNC-S hydrogel showed sustained release (until 8 weeks) of VEGF whereas CNC-DS showed a very fast release of VEGF with a large burst. Subcutaneously injected CNC-S hydrogel showed much enhanced cellular infiltration as well as better biocompatibility with milder foreign body response than CNC-DS hydrogel. Furthermore, VEGF-loaded CNC-S hydrogel induced significant angiogenesis inside the hydrogel whereas VEGF-loaded CNC-DS did not.

Conclusion: CNC-S possesses good properties as a biomaterial including injectability, biocompatibility, and allowing cellular infiltration and sustained release of growth factors. VEGF-loaded CNC-S hydrogel exhibited efficient angiogenesis inside the hydrogel. The sulfate group of CNC-S was a key for good biocompatibility and the biological activities of VEGF-loaded CNC hydrogel.

Keywords: Angiogenesis; Cellular infiltration; Cellulose nanocrystal (CNC); Injectable hydrogel; Vascular endothelial growth factor.

Grants and funding

2021R1A2C2004722/NRF (KR)