A self-developed AIM-InDel panel designed for degraded DNA analysis: Forensic application characterization and genetic landscape investigation in the Han Chinese population

Qiong Lan; Shuanglin Li; Meiming Cai; Fanzhang Lei; Chunmei Shen; Bofeng Zhu

doi:10.1016/j.ygeno.2023.110620

A self-developed AIM-InDel panel designed for degraded DNA analysis: Forensic application characterization and genetic landscape investigation in the Han Chinese population

Genomics. 2023 May;115(3):110620. doi: 10.1016/j.ygeno.2023.110620. Epub 2023 Apr 8.

Authors

Qiong Lan¹, Shuanglin Li², Meiming Cai³, Fanzhang Lei³, Chunmei Shen⁴, Bofeng Zhu⁵

Affiliations

¹ Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China.
² Department of Anatomy and Histology, School of Basic Medical Sciences, Shenzhen University, Shenzhen, China.
³ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China.
⁴ Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address: cmshen2004@126.com.
⁵ Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China. Electronic address: zhubofeng7372@126.com.

PMID: 37037275
DOI: 10.1016/j.ygeno.2023.110620

Abstract

To assist in forensic DNA investigation, we developed a new panel capable of simultaneously amplifying 56 ancestry-informative InDels, three Y-InDels and the Amelogenin locus in one PCR reaction. The fragment lengths of the InDel amplicons in this panel were restricted to <200 bp to benefit degraded DNA analysis. In this study, we explored the efficiency of this new panel for forensic applications in the Han Chinese population, and further shed light on the genetic structures of Han populations. We showed that the new panel could be served as an efficient tool for ancestry inference of intercontinental populations. Especially, the Han individuals in different regions could be 100% correctly predicted to be of East Asian origin with this new panel. The Han populations in different regions shared similar ancestry components in their genetic structures. Besides, we also revealed that the new panle could be useful for individual identification in different Han Chinese populations. In conclusion, we have provided the necessary evidence that the self-constructed new panel could play an important role in forensic DNA investigation.

Keywords: Ancestry inference; Capillary electrophoresis; Degraded DNA; Forensic genetics; Self-developed panel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA
East Asian People*
Gene Frequency
Genetics, Population*
Humans

Substances

DNA